7BB3
Structure of S. pombe YG-box oligomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2016-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976251 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 27.190, 83.710, 160.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.493 - 2.158 |
| R-factor | 0.2586 |
| Rwork | 0.257 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rg5 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.1_3469) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.493 | 2.235 |
| High resolution limit [Å] | 2.158 | 2.158 |
| Rmerge | 0.105 | 0.824 |
| Rmeas | 0.114 | 0.891 |
| Rpim | 0.045 | 0.334 |
| Number of reflections | 10282 | 1010 |
| <I/σ(I)> | 10.85 | 2.25 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.998 | 0.708 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 289.15 | 65 % 2-methylpentanediol, 80 mM KCl, 40 mM HEPES |
