7BAE
Crystal structure of PAFB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-21 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.98013 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.124, 36.953, 24.878 |
| Unit cell angles | 90.00, 103.84, 90.00 |
Refinement procedure
| Resolution | 31.500 - 1.200 |
| R-factor | 0.2123 |
| Rwork | 0.211 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6haj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | Aimless |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.510 | 1.200 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 16905 | 799 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 98.3 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.997 | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293.15 | 0.7 mM Palmitic acid, 0.1 M Potassium phosphate citrate pH 4.2, 40 % PEG 300 |
