7BA8
Cys-42-tethered stabilizer 10 of 14-3-3(sigma)/ERa PPI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-04-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.702, 112.163, 62.269 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.305 - 1.200 |
R-factor | 0.1864 |
Rwork | 0.185 |
R-free | 0.20670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jc3 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3139) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 66.040 | 66.040 | 1.220 |
High resolution limit [Å] | 1.200 | 6.460 | 1.200 |
Rmerge | 0.064 | 0.041 | 1.159 |
Rmeas | 0.067 | 0.042 | 1.288 |
Rpim | 0.019 | 0.011 | 0.536 |
Total number of observations | 1064974 | 8393 | 22511 |
Number of reflections | 89026 | 655 | 4245 |
<I/σ(I)> | 18.2 | 61.2 | 1.4 |
Completeness [%] | 99.6 | 99.9 | 94.3 |
Redundancy | 12 | 12.8 | 5.3 |
CC(1/2) | 0.999 | 0.999 | 0.535 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 277 | 0.095 M HEPES, 0.19 M CaCl2, 27% (v/v) PEG400, 5% (v/v) glycerol |