7BA2
D319A mutant of the PilB minor pilin from Streptococcus sanguinis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 61 |
| Unit cell lengths | 120.950, 120.950, 151.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.310 - 3.000 |
| Rwork | 0.241 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7b7p |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.270 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.630 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rpim | 0.667 | |
| Number of reflections | 25101 | 1251 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 8.3 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-tris (pH 6.5), 3 M NaCl |
