7B9Q
The SERp optimized structure of Ribonucleotide reductase from Rhodobacter sphaeroides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 43 |
Unit cell lengths | 161.588, 161.588, 97.308 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.588 - 2.780 |
R-factor | 0.222 |
Rwork | 0.220 |
R-free | 0.27070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7b9p |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.588 | 46.588 | 2.940 |
High resolution limit [Å] | 2.776 | 8.220 | 2.780 |
Rmerge | 0.197 | 0.033 | 2.509 |
Rmeas | 0.212 | 0.035 | 2.695 |
Number of reflections | 63329 | 2525 | 10055 |
<I/σ(I)> | 9.63 | 49.22 | 0.77 |
Completeness [%] | 99.8 | 99.4 | 98.9 |
Redundancy | 7.641 | 7.281 | 7.408 |
CC(1/2) | 0.997 | 0.999 | 0.293 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Precipitant: 40% MPD, 5% PEG 8000, 100 mM MES-Bufffer Protein sample: 25 mg/mL Enzyme + 100 uM dATP Ratio 1:1 |