7B9P

Structure of Ribonucleotide reductase from Rhodobacter sphaeroides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 65 2 2
Unit cell lengths	140.748, 140.748, 364.277
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.860 - 2.646
R-factor	0.2312
Rwork	0.230
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ensemble
Data reduction software	XDS
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.860	2.740
High resolution limit [Å]	2.646	2.650
Rmerge	0.169	7.684
Rmeas	0.174	7.886
Rpim	0.040	1.756
Number of reflections	62949	6078
<I/σ(I)>	13.9	0.3
Completeness [%]	99.8	98.4
Redundancy	19.29	19.7
CC(1/2)	0.999	0.148

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	Precipitant: 30% PEG 1500, 20% Glycerol Protein solution: 45 mg/mL Protein + 100 uM dATP Hanging drop experiment: 1.0 uL Protein solution + 1.0 uL Precipitant over 500 uL precipitant