7B96
NHL domain of human TRIM2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.872900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.180, 83.200, 132.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.700 - 1.800 |
R-factor | 0.1782 |
Rwork | 0.177 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qf7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.082 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.700 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.138 | 1.094 |
Rmeas | 0.155 | 1.240 |
Rpim | 0.070 | 0.571 |
Number of reflections | 46050 | 4531 |
<I/σ(I)> | 6.72 | 1.24 |
Completeness [%] | 99.5 | |
Redundancy | 4.5 | |
CC(1/2) | 0.990 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10mg/mL protein in 20mM Tris pH 7.5, 150mM NaCl, 20% (v/v) glycerol and 1mM DTT was added at a 1:1 ratio to the reservoir buffer: 0.2M potassium chloride and 24% (w/v) polyethylene glycol 3350 pH 7.5 |