7B8V
Circular permutant of ribosomal protein S6, P54-55
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.450, 73.630, 131.008 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.940 - 1.863 |
| R-factor | 0.2487 |
| Rwork | 0.247 |
| R-free | 0.28620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.940 | 48.940 | 2.071 |
| High resolution limit [Å] | 1.850 | 6.003 | 1.863 |
| Rmerge | 0.113 | 0.051 | 0.793 |
| Rmeas | 0.126 | 0.058 | 0.893 |
| Rpim | 0.055 | 0.026 | 0.404 |
| Total number of observations | 9571 | 8651 | |
| Number of reflections | 147738 | 1945 | 1947 |
| <I/σ(I)> | 8.6 | 21 | 1.7 |
| Completeness [%] | 93.8 | 98.6 | 79.3 |
| Redundancy | 5.2 | 4.9 | 4.4 |
| CC(1/2) | 0.994 | 0.999 | 0.464 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M sodium bromide 0.1 M Bis-Tris propane pH 6.5 20% w/v PEG 3350 |
