7B8S

Fusidic acid bound structure of bacterial efflux pump.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	109.709, 145.237, 175.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.831 - 2.300
R-factor	0.2028
Rwork	0.201
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5en5
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.831	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	124264	12209
<I/σ(I)>	12.67
Completeness [%]	99.7
Redundancy	9
CC(1/2)	0.997	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M MES pH 6.5, 0.21 M NaCl, 11.5% PEG4000