7B6C
BK Polyomavirus VP1 pentamer fusion with long C-terminal extended arm
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976251 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 234.297, 97.205, 146.166 |
Unit cell angles | 90.00, 98.38, 90.00 |
Refinement procedure
Resolution | 90.000 - 2.484 |
Rwork | 0.205 |
R-free | 0.23330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mj0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.574 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 2.570 |
High resolution limit [Å] | 2.480 | 2.480 |
Rpim | 0.111 | |
Number of reflections | 113999 | 11185 |
<I/σ(I)> | 6.29 | |
Completeness [%] | 99.0 | 98.22 |
Redundancy | 3.7 | |
CC(1/2) | 0.984 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 33 % EDO_P8K,100 mM Morpheus buffer 1 (pH 6.5), 50 mM Morpheus NPS |