Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.976800 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 138.622, 149.719, 65.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.440 |
| Rwork | 0.162 |
| R-free | 0.18580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mj0 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.851 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rpim | 0.029 | 0.562 |
| Number of reflections | 245801 | 24335 |
| <I/σ(I)> | 16.4 | 1.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 13.2 | 11.8 |
| CC(1/2) | 0.999 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 10% PEG 3350, 0.2 M NaJ, 0.1 M bis-tris pH 5.5 |
