7B5U

RCK_C domain dimer of S.agalactiae BusR in complex with c-di-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	33.164, 60.947, 35.714
Unit cell angles	90.00, 101.73, 90.00

Refinement procedure

Resolution	17.670 - 1.200
R-factor	0.132
Rwork	0.131
R-free	0.15530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7b5t
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.670	1.230
High resolution limit [Å]	1.200	1.200
Rmeas	0.034	0.110
Number of reflections	41497	2859
<I/σ(I)>	33.3	14.86
Completeness [%]	95.4
Redundancy	6.9
CC(1/2)	0.990	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	100 mM Na-acetate, 3% (w/v) PEG4000