7B5T
S. agalactiae BusR transcription factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9797 |
Spacegroup name | I 4 |
Unit cell lengths | 114.180, 114.180, 240.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.220 - 2.800 |
R-factor | 0.2579 |
Rwork | 0.257 |
R-free | 0.26890 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.220 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 74011 | 3694 |
<I/σ(I)> | 19.4 | |
Completeness [%] | 99.1 | |
Redundancy | 6.9 | |
CC(1/2) | 1.000 | 0.615 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 150 mM Mg-acetate, 6% D+ Trehalose |