7B4O
A Bacteroidetes bacterium CuZn-superoxide dismutase with ZnZn metalation
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.469, 96.637, 136.118 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.270 - 1.410 |
| Rwork | 0.159 |
| R-free | 0.19180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.358 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.270 | 1.430 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Number of reflections | 122059 | 5982 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.5 | |
| CC(1/2) | 0.998 | 0.345 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 100 mM tris(hydroxymethyl)aminomethane-HCl, 20 % polyethylene glycol 6000, 2 mM ZnCl2 |
