7B2O

Crystal structure of Chlamydomonas reinhardtii chloroplastic sedoheptulose-1,7-bisphosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.980113458075
Spacegroup name	P 21 21 2
Unit cell lengths	178.224, 183.652, 75.196
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.470 - 3.090
R-factor	0.1953
Rwork	0.193
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iz3
RMSD bond length	0.005
RMSD bond angle	0.737
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.470	3.200
High resolution limit [Å]	3.090	3.090
Number of reflections	45740	4245
<I/σ(I)>	12.02	1.72
Completeness [%]	99.3	94.2
Redundancy	13.4	12.9
CC(1/2)	0.983	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Sodium HEPES pH 7.5; 2% (v/v) PEG 400; 2.0 M Ammonium sulfate