7B28

Complement inhibitor CirpA3 from Rhipicephalus pulchellus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	68.366, 150.298, 68.430
Unit cell angles	90.00, 91.83, 90.00

Refinement procedure

Resolution	62.250 - 2.100
R-factor	0.2218
Rwork	0.217
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CirpA1
RMSD bond length	0.003
RMSD bond angle	0.531
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18rc4_3812)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.330	2.140
High resolution limit [Å]	2.100	2.110
Number of reflections	78899	11656
<I/σ(I)>	5.1
Completeness [%]	99.0
Redundancy	3.4
CC(1/2)	0.991	0.471

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium sulphate, 30% (w/v) PEG 4000