7B1W
Crystal structure of plastidial ribulose epimerase RPE1 from the model alga Chlamydomonas reinhardtii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-17 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.293, 297.322, 77.747 |
Unit cell angles | 90.00, 116.30, 90.00 |
Refinement procedure
Resolution | 49.289 - 1.935 |
R-factor | 0.1846 |
Rwork | 0.184 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Homology model |
RMSD bond length | 0.008 |
RMSD bond angle | 0.877 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.290 | 2.005 |
High resolution limit [Å] | 1.935 | 1.935 |
Number of reflections | 229414 | 21621 |
<I/σ(I)> | 9.79 | |
Completeness [%] | 98.7 | |
Redundancy | 7.2 | |
CC(1/2) | 0.997 | 0.616 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Calcium acetate 160 mmol/L Sodium cacodylate pH 6.5 80 mmol/L Glycerol 20 % Polyethylene glycol 8000 14.4 % |