7B1N
Crystal structure of phosphatidyl serine synthase (PSS) in the closed conformation with bound citrate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 62.130, 70.810, 94.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.700 - 2.800 |
| R-factor | 0.305 |
| Rwork | 0.302 |
| R-free | 0.35470 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.744 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc5_4047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.243 | 1.920 |
| Rmeas | 0.266 | 2.110 |
| Rpim | 0.107 | 0.864 |
| Number of reflections | 19466 | 1017 |
| <I/σ(I)> | 4.51 | 0.86 |
| Completeness [%] | 99.1 | 97.6 |
| Redundancy | 6.2 | 5.7 |
| CC(1/2) | 0.989 | 0.578 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 300 | Qiagens MBclass Suit and MBclass Suit II Molecular Dimension MemGold |
