7B1L
Crystal structure of phosphatidyl serine synthase (PSS) in the closed conformation with bound citrate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 61.630, 79.480, 95.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.2649 |
| Rwork | 0.263 |
| R-free | 0.30100 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc5_4047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.106 | 1.400 |
| Rmeas | 0.116 | 1.570 |
| Rpim | 0.050 | 0.660 |
| Number of reflections | 39735 | 3636 |
| <I/σ(I)> | 936 | 1.35 |
| Completeness [%] | 97.5 | 95.6 |
| Redundancy | 6.1 | 5.3 |
| CC(1/2) | 0.999 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 300 | Qiagens MBclass Suit and MBclass Suit II Molecular Dimension MemGold |
