7B1J
Orthorhombic P21212 Structure of Human Mad1 C-terminal Domain in Complex with Phosphorylated Bub1 CD1 Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 87.680, 133.980, 34.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.750 - 2.900 |
R-factor | 0.263 |
Rwork | 0.261 |
R-free | 0.29610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dzo |
RMSD bond length | 0.025 |
RMSD bond angle | 2.330 |
Data reduction software | xia2 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.750 | 3.500 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.033 | 0.469 |
Rmeas | 0.036 | 0.510 |
Rpim | 0.014 | 0.020 |
Number of reflections | 9638 | 454 |
<I/σ(I)> | 25.9 | 3.9 |
Completeness [%] | 100.0 | 99.6 |
Redundancy | 3.6 | 3.6 |
CC(1/2) | 1.000 | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% Isopropanol, 0.1 M Na HEPES, pH 7.5, 20% PEG 4000 |