7B1H
Monoclinic P21 Structure of Human Mad1 C-terminal Domain in Complex with Phosphorylated Bub1 CD1 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.630, 132.340, 82.750 |
| Unit cell angles | 90.00, 93.34, 90.00 |
Refinement procedure
| Resolution | 39.430 - 2.400 |
| R-factor | 0.2337 |
| Rwork | 0.231 |
| R-free | 0.28060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dzo |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.905 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.430 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.060 |
| Rmeas | 0.070 | 0.070 |
| Rpim | 0.040 | 0.040 |
| Number of reflections | 100346 | 4893 |
| <I/σ(I)> | 14.5 | 1.7 |
| Completeness [%] | 99.6 | 99.04 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 1.000 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 8% Isopropanol, 20% PEG 4000, 0.1 M Na HEPES, pH 7.5 |
