7B1F

Orthorhombic P212121 Structure of Human Mad1 C-terminal Domain in Complex with Phosphorylated Bub1 CD1 Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	34.219, 80.492, 133.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.250 - 1.750
R-factor	0.2573
Rwork	0.256
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dzo
Data reduction software	xia2
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.250	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.050	1.970
Rmeas	0.060	2.300
Rpim	0.020	1.200
Number of reflections	33937	1863
<I/σ(I)>	16
Completeness [%]	89.0
Redundancy	5.6
CC(1/2)	1.000	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 4000, 0.1 M Hepes pH 7.5, 10% isopropanol