7B19

Mutant Myosin-II-GGG motor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	C 2 2 21
Unit cell lengths	89.187, 149.031, 153.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.590 - 2.550
R-factor	0.2086
Rwork	0.206
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vom
RMSD bond length	0.011
RMSD bond angle	1.124
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.590	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	33626	3315
<I/σ(I)>	9.77	2.09
Completeness [%]	99.5
Redundancy	2
CC(1/2)	0.997	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.24 M sodium malonate, pH8.0, 27% PEG 3350