7B0O
In meso structure of the membrane integral lipoprotein intramolecular transacylase Lit from Bacillus cereus in space group P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96862 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.724, 44.700, 116.865 |
Unit cell angles | 90.00, 92.38, 90.00 |
Refinement procedure
Resolution | 43.320 - 2.330 |
R-factor | 0.2472 |
Rwork | 0.245 |
R-free | 0.28730 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.760 | 2.450 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmeas | 0.290 | 1.450 |
Number of reflections | 24031 | 1202 |
<I/σ(I)> | 3.4 | 1.4 |
Completeness [%] | 91.5 | 56.6 |
Redundancy | 6.2 | 6.9 |
CC(1/2) | 0.960 | 0.180 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 293 | 100 mM sodium citrate/HCl, pH 5.6, 40 %(v/v) polyethylene glycol (PEG) 400, 100 mM ammonium sulfate, and 200 mM sodium formate |
2 |