7B0B

Fab HbnC3t1p1_C6 bound to SARS-CoV-2 RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-09-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	84.413, 109.109, 168.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.360 - 2.980
R-factor	0.212
Rwork	0.209
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bz5 Fb C6
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_3965)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.360	3.080
High resolution limit [Å]	2.980	2.980
Rmeas	0.163
Number of reflections	25113	537
<I/σ(I)>	4.1
Completeness [%]	77.3
Redundancy	7
CC(1/2)		0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M Tris pH8.5, 20% poly ethylene glycol 6000 using 14 mg/ml protein in a 1.3:1 protein to reservoir ratio