7B08
TgoT apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-01-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.972 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.060, 105.220, 152.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.860 - 2.394 |
R-factor | 0.2416 |
Rwork | 0.241 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tgo |
RMSD bond length | 0.005 |
RMSD bond angle | 0.750 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.860 | 2.480 |
High resolution limit [Å] | 2.394 | 2.394 |
Rmerge | 0.122 | 4.034 |
Number of reflections | 41950 | 3986 |
<I/σ(I)> | 11.45 | |
Completeness [%] | 99.3 | |
Redundancy | 2.5 | |
CC(1/2) | 0.999 | 0.287 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG 20K and 0.1 M MES-NaOH pH 6.5 |