7B06
TgoT_RT521 apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2018-06-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.530, 109.850, 111.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.690 - 2.349 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tgo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.750 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.690 | 2.434 |
| High resolution limit [Å] | 2.340 | 2.350 |
| Rmerge | 0.099 | 1.565 |
| Number of reflections | 36488 | 3526 |
| <I/σ(I)> | 7.69 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.997 | 0.486 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 400, 0.1 M sodium acetate pH4.6 and 0.2M MgCl2 |
