7B01
ADAMTS13-CUB12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 105.576, 105.576, 125.837 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.000 - 2.800 |
| R-factor | 0.2053 |
| Rwork | 0.201 |
| R-free | 0.29170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6i58 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.505 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.110 | 2.700 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Number of reflections | 86169 | 7612 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 92.2 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.990 | 0.084 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 21% (v/v) PEG1500, 100 mM Sodium propionate, sodium cacodylate trihydrate, 200 mM MnCl2, Bis-Tris propane, pH 5 |
