7AZT
X-ray crystallographic structure of (6-4)photolyase from Drosophila melanogaster at room temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2019-05-19 |
Detector | MPCCD |
Wavelength(s) | 1.7712 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.630, 91.640, 115.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.610 - 2.270 |
R-factor | 0.1742 |
Rwork | 0.172 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ayv |
RMSD bond length | 0.007 |
RMSD bond angle | 0.886 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.610 | 2.351 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 33953 | 1352 |
<I/σ(I)> | 4.93 | |
Completeness [%] | 100.0 | |
Redundancy | 106 | |
CC(1/2) | 0.970 | 0.320 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6.5 | 277.15 | 0.2 M lithium sulftate, 0.1 M bis-tris (pH=6.5), 25% PEG3350, 4% polypropyle glycol P400 |