7AZL

DNA polymerase sliding clamp from Escherichia coli with peptide 38 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	139.611, 86.643, 153.897
Unit cell angles	90.00, 92.56, 90.00

Refinement procedure

Resolution	70.690 - 2.420
R-factor	0.21
Rwork	0.208
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fvl
RMSD bond length	0.012
RMSD bond angle	1.527
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.690	2.460
High resolution limit [Å]	2.420	2.420
Rpim	0.051	0.390
Number of reflections	70023	3408
<I/σ(I)>	11.3	2.1
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.2 M Sodium formate pH 7.2, PEG 3350 20% (w/v)