7AZG

DNA polymerase sliding clamp from Escherichia coli with peptide 4 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	70.422, 165.302, 138.510
Unit cell angles	90.00, 91.84, 90.00

Refinement procedure

Resolution	33.650 - 2.920
R-factor	0.187
Rwork	0.184
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fvl
RMSD bond length	0.007
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.650	3.220
High resolution limit [Å]	2.920	2.920
Rpim		0.814
Number of reflections	46885	2342
<I/σ(I)>	6.4	1.5
Completeness [%]	93.2
Redundancy	7
CC(1/2)	0.980	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.2 M Succinic acid pH 7.0, PEG 3350 20% (w/v)