7AZC
DNA polymerase sliding clamp from Escherichia coli with peptide 22 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.809, 76.785, 79.806 |
| Unit cell angles | 70.90, 68.22, 89.14 |
Refinement procedure
| Resolution | 25.590 - 1.770 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fvl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.020 | 1.930 |
| High resolution limit [Å] | 1.770 | 1.780 |
| Rpim | 0.044 | 0.550 |
| Number of reflections | 107545 | 5173 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 90.1 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.998 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 2% v/v Tacsimate pH 4.0, 0.1M Sodium acetate trihydrate pH 4.6, PEG 3350 16% (w/v) |
