7AZB

Structure of DDR2 DS domain in complex with VHH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-20
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.98009
Spacegroup name	P 31 2 1
Unit cell lengths	63.244, 63.244, 120.854
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.890 - 2.620
R-factor	0.214
Rwork	0.210
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z4f
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BUSTER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.770	2.740
High resolution limit [Å]	2.620	2.620
Number of reflections	8157	408
<I/σ(I)>	10.6
Completeness [%]	92.4
Redundancy	10.9
CC(1/2)	0.997	0.468

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M lithium sulphate monohydrate, 20 % PEG3350