7AZ8
DNA polymerase sliding clamp from Escherichia coli with peptide 43 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 63.806, 64.731, 70.676 |
Unit cell angles | 106.54, 99.87, 117.25 |
Refinement procedure
Resolution | 54.740 - 1.610 |
R-factor | 0.179 |
Rwork | 0.178 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fvl |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.740 | 1.630 |
High resolution limit [Å] | 1.610 | 1.610 |
Rpim | 0.036 | 1.187 |
Number of reflections | 87336 | 68 |
<I/σ(I)> | 13 | 0.7 |
Completeness [%] | 88.2 | |
Redundancy | 6.8 | |
CC(1/2) | 0.990 | 0.260 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.2 M DL Malic acid pH 7, PEG 3350 20% (w/v) |