7AZ5

DNA polymerase sliding clamp from Escherichia coli with peptide 47 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	68.886, 80.166, 83.371
Unit cell angles	62.79, 71.29, 81.66

Refinement procedure

Resolution	27.350 - 1.870
R-factor	0.179
Rwork	0.177
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fvl
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.240	2.030
High resolution limit [Å]	1.870	1.870
Rpim	0.135	0.526
Number of reflections	90577	4529
<I/σ(I)>	11.1	1.1
Completeness [%]	93.5
Redundancy	7
CC(1/2)	0.990	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.2 M Sodium formate pH 7.2, PEG 3350 20% (w/v)