7AZ0
Structure of the mouse 8-oxoguanine DNA Glycosylase mOGG1 in complex with TH12161
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-29 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.086, 81.645, 169.631 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.676 - 2.400 |
Rwork | 0.230 |
R-free | 0.25660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6g3y |
RMSD bond length | 0.004 |
RMSD bond angle | 1.249 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.640 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 44894 | 4638 |
<I/σ(I)> | 11.1 | 1.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 26.3 | |
CC(1/2) | 0.999 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.12 M Alcohols, 0.1 M Buffer System 2 pH 7.5, 30% v/v Precipitant Mix 2 (Morpheus Screen, Molecular Dimensions) |