7AYV
X-ray crystallographic structure of (6-4)photolyase from Drosophila melanogaster at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.903, 88.933, 114.022 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.420 - 1.790 |
| R-factor | 0.1771 |
| Rwork | 0.176 |
| R-free | 0.20620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cvy |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.205 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1-3660-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.420 | 1.854 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 58656 | 3836 |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 99.2 | |
| Redundancy | 12.7 | |
| CC(1/2) | 0.990 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.2 M Lithium sulphate, 0.1 M Bis-Tris(pH=6.5), 25%PEG3350, 4% polypropylene glycol p 400 |
