7AY3
Crystal structure of the CBM36-1 domain of a multidomain xylanase from the hindgut metagenome of Trinervitermes trinervoides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-31 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.432, 37.320, 108.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.616 - 2.000 |
| R-factor | 0.182296205054 |
| Rwork | 0.175 |
| R-free | 0.24911 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c71 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.785 |
| Data reduction software | MOSFLM (1.12_2829) |
| Data scaling software | SCALA (1.12_2829) |
| Phasing software | PHASER (1.12_2829) |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.160 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 8260 | 715 |
| <I/σ(I)> | 22 | |
| Completeness [%] | 98.3 | 87 |
| Redundancy | 2 | |
| CC(1/2) | 0.999 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | PEG 3000, Hepes |
