7AY3

Crystal structure of the CBM36-1 domain of a multidomain xylanase from the hindgut metagenome of Trinervitermes trinervoides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-31
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	28.432, 37.320, 108.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.616 - 2.000
R-factor	0.182296205054
Rwork	0.175
R-free	0.24911
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c71
RMSD bond length	0.006
RMSD bond angle	0.785
Data reduction software	MOSFLM (1.12_2829)
Data scaling software	SCALA (1.12_2829)
Phasing software	PHASER (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.160	2.060
High resolution limit [Å]	1.990	1.990
Number of reflections	8260	715
<I/σ(I)>	22
Completeness [%]	98.3	87
Redundancy	2
CC(1/2)	0.999	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	PEG 3000, Hepes