7AY2

Crystal structure of truncated USP1-UAF1 reacted with ubiquitin-prg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	134.242, 134.242, 274.673
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.120 - 3.200
R-factor	0.2058
Rwork	0.204
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cvn 5cvl
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.560	3.310
High resolution limit [Å]	3.200	3.200
Rmeas	0.193	1.657
Rpim	0.059	0.519
Number of reflections	46123	4501
<I/σ(I)>	11.6	1.7
Completeness [%]	100.0	100
Redundancy	10.5	10.1
CC(1/2)	0.997	0.581

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	8-13% w/v PEG3350, 0.1 M citric acid/Bis-Tris propane pH 4.1