7AX5

Anammox-specific acyl carrier protein from Kuenenia stuttgartiensis; ensemble refinement

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 64
Unit cell lengths	76.810, 76.810, 30.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.404 - 1.756
R-factor	0.1776
Rwork	0.175
R-free	0.23430
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.ensemble_refinement:1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.404	1.900
High resolution limit [Å]	1.756	1.760
Rpim	0.025	0.652
Number of reflections	7492	1132
<I/σ(I)>	16.4
Completeness [%]	91.6
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	28% (v/v) PEG 400, 200 mM calcium acetate, 100 mM sodium acetate pH 4.5 and 10 mM zinc chloride