7AVF
Triclinic hydrogenated hen egg-white lysozyme at 100 K (control)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-15 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.7293 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.760, 31.070, 33.770 |
| Unit cell angles | 89.21, 72.46, 67.86 |
Refinement procedure
| Resolution | 31.990 - 1.000 |
| R-factor | 0.1182 |
| Rwork | 0.117 |
| R-free | 0.13190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yeo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.464 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.990 | 1.036 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.073 | 0.386 |
| Rmeas | 0.086 | 0.453 |
| Rpim | 0.045 | 0.236 |
| Number of reflections | 50297 | 4908 |
| <I/σ(I)> | 9.25 | 2.81 |
| Completeness [%] | 97.5 | 94.95 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.996 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4.5 | 291 | sodium nitrate, sodium acetate pH 4.5 |
