7AV6

FAST in a domain-swapped dimer form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9772
Spacegroup name	P 41 21 2
Unit cell lengths	45.920, 45.920, 105.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.080 - 1.500
R-factor	0.2153
Rwork	0.214
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1odv
RMSD bond length	0.005
RMSD bond angle	0.736
Data reduction software	XDS (20200131)
Data scaling software	XSCALE (20200131)
Phasing software	MoRDa (1.3.02)
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.090	42.090	1.540
High resolution limit [Å]	1.500	3.610	1.500
Rpim	0.007	0.006	0.726
Number of reflections	18549	1494	1274
<I/σ(I)>	32.8	103.7	0.7
Completeness [%]	98.5	99.9	96.6
Redundancy	25.4	23	23.9
CC(1/2)	1.000	1.000	0.325

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M magnesium formate, 20 % w/v PEG 3350