7AV4
Dark state structure of the C432S mutant of Fatty Acid Photodecarboxylase (FAP)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.375, 104.683, 156.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.731 - 1.936 |
| Rwork | 0.175 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yru |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.649 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.956 | 2.050 |
| High resolution limit [Å] | 1.936 | 1.936 |
| Rmeas | 0.097 | |
| Number of reflections | 56924 | 8961 |
| <I/σ(I)> | 10.8 | 1 |
| Completeness [%] | 99.4 | 98.1 |
| Redundancy | 3.8 | 3.6 |
| CC(1/2) | 0.998 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | ~ 20-30 % PEG 4000, 0.1 M Citrate pH5.5, 10 mM spermidine |
