7AUR
Yeast Diphosphoinositol Polyphosphate Phosphohydrolase DDP1 in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979300 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.958, 61.958, 95.924 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.870 - 1.650 |
R-factor | 0.2106 |
Rwork | 0.209 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7aui |
RMSD bond length | 0.007 |
RMSD bond angle | 1.512 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.960 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.083 | 0.666 |
Rpim | 0.020 | 0.164 |
Number of reflections | 26240 | 1299 |
<I/σ(I)> | 18.9 | 4.8 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 19 | 17.2 |
CC(1/2) | 0.999 | 0.937 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 25% PEG 6000, 0.1 M Sodium acetate pH 5.0, 0.1 M NaCl. Protein:precipitant ratio 2:1. Protein buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 1 mM DTT, 10 mM AMP-PNP. |