7AUP
Yeast Diphosphoinositol Polyphosphate Phosphohydrolase DDP1 in complex with PCP-InsP7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979260 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.702, 61.702, 95.844 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.720 - 1.850 |
R-factor | 0.2258 |
Rwork | 0.223 |
R-free | 0.26820 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 7aun |
RMSD bond length | 0.011 |
RMSD bond angle | 1.826 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.720 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.052 | 0.652 |
Rpim | 0.013 | 0.160 |
Number of reflections | 18625 | 1141 |
<I/σ(I)> | 33.2 | 6.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.6 | 17.9 |
CC(1/2) | 1.000 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 20% PEG 3350, 0.1 M Sodium acetate pH 4.5, 0.1 M NaCl. Protein buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 1 mM DTT, 10 mM PCP-InsP7. |