7AUO
Yeast Diphosphoinositol Polyphosphate Phosphohydrolase DDP1 in complex with PA-InsP8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979260 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.674, 61.674, 89.219 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.870 - 2.650 |
R-factor | 0.2359 |
Rwork | 0.234 |
R-free | 0.27150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7aui |
RMSD bond length | 0.005 |
RMSD bond angle | 1.348 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.870 | 2.780 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.058 | 0.650 |
Rpim | 0.014 | 0.152 |
Number of reflections | 6055 | 799 |
<I/σ(I)> | 25.1 | 5.3 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 18 | 19.1 |
CC(1/2) | 1.000 | 0.972 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 25% PEG 6000, 0.1 M Sodium acetate pH 5.0, 0.1 M NaCl. Protein:precipitant ratio 2:1. Protein buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 1 mM DTT, 10 mM PA-InsP8. |