7ASW

Crystal structure of chloroplastic thioredoxin z defines a novel type-specific target recognition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.983995
Spacegroup name	P 32 2 1
Unit cell lengths	61.985, 61.985, 61.138
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.990 - 2.444
Rwork	0.227
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i1c
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.990	2.530
High resolution limit [Å]	2.440	2.440
Number of reflections	5268	485
<I/σ(I)>	27.43	2.04
Completeness [%]	99.4
Redundancy	39.1
CC(1/2)	0.997	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.1	1.26 mol.L-1 ammonium sulfate 0,1 mol.L-1 HEPES pH=7.5