7ARN
Crystal Structure of the Fab Fragment of a Glycosylated Lymphoma Antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-06-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.550, 60.580, 82.450 |
Unit cell angles | 90.00, 111.91, 90.00 |
Refinement procedure
Resolution | 44.320 - 1.570 |
R-factor | 0.1965 |
Rwork | 0.195 |
R-free | 0.23220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6i9i |
RMSD bond length | 0.006 |
RMSD bond angle | 0.895 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.320 | 1.626 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.055 | 0.805 |
Rmeas | 0.065 | 0.962 |
Rpim | 0.035 | 0.520 |
Number of reflections | 59090 | 5799 |
<I/σ(I)> | 9.95 | 1.09 |
Completeness [%] | 96.7 | 95.31 |
Redundancy | 3.5 | 3.2 |
CC(1/2) | 0.998 | 0.575 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 30% Jeffamine ED-2001 Reagent pH 7.0 0.1M HEPES pH 7.0 8% 2,5 hexanediol |