7AQZ

Co-Crystal Structure of Variant Surface Glycoprotein VSG2 in complex with Nanobody VSG2(NB14)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-19
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	92.542, 95.950, 124.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.237 - 1.300
R-factor	0.1522
Rwork	0.151
R-free	0.17230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vsg 5lhr(chainB)
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.240	1.346
High resolution limit [Å]	1.300	1.300
Number of reflections	266024	23312
<I/σ(I)>	17.72
Completeness [%]	98.3
Redundancy	12.3
CC(1/2)	1.000	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	0.1 M citrate, 23 % PEG 1500