7AQX

Co-Crystal Structure of Variant Surface Glycoprotein VSG2 in complex with Nanobody VSG2(NB9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-28
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	94.088, 95.565, 123.721
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.782 - 1.499
R-factor	0.1654
Rwork	0.164
R-free	0.19160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vsg 5lhr(ChainB)
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX (1.15.2_3472)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.782	1.553
High resolution limit [Å]	1.499	1.499
Number of reflections	11913750	17100
<I/σ(I)>	51.47
Completeness [%]	99.2
Redundancy	67.3
CC(1/2)	0.992	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	20 % PEG 1500, 0.1 M citrate